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rat anti mouse cd16 32 clone 2 4g2  (Bio X Cell)


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    Bio X Cell rat anti mouse cd16 32 clone 2 4g2
    Rat Anti Mouse Cd16 32 Clone 2 4g2, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+4g2/pm41742422-271-18-23?v=Bio+X+Cell
    Average 98 stars, based on 730 article reviews
    rat anti mouse cd16 32 clone 2 4g2 - by Bioz Stars, 2026-07
    98/100 stars

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    Gating strategy for the identification of murine CD127 + and CD127 − NK cell subsets. Sequential flow cytometric gating strategy used to identify and characterize murine NK cells from single-cell suspensions of spleen tissue. ( a ) Lymphocyte gate: Representative flow cytometry plots illustrating the initial electronic gate set on the small lymphocyte population based on forward scatter (FSC) and side scatter (SSC) characteristics following red blood cell lysis and Fc receptor blocking with 2.4G2 antibody. ( b ) Singlet discrimination: Doublets were excluded by FSC-A versus FSC-H gating to ensure analysis of single cells. ( c ) NK cell identification: Within the lymphocyte gate, NK cells were identified as NK1.1 + cells negative for T cell markers (CD3, TCRβ) and B cell markers (CD19), yielding a purified NK cell population defined as NK1.1 + CD3 − TCRβ − CD19 − cells. CD122 expression was consistently detected within this gate, further confirming NK cell identity. ( d ) Subset characterization: Representative flow cytometric plots showing differential expression of CD127 and additional maturation and receptor markers, such as Mac-1, CD43, Ly49D, NKG2A/C/E, CD27, and CD11c, within the CD127 + and CD127 − NK cell subsets. Numbers in quadrants indicate the percentage of cells within each region.

    Journal: International Journal of Molecular Sciences

    Article Title: CD127 + Natural Killer Cells Represent a Distinct, Interleukin-15-Independent and Thymus-Independent Subset in Mice

    doi: 10.3390/ijms27062667

    Figure Lengend Snippet: Gating strategy for the identification of murine CD127 + and CD127 − NK cell subsets. Sequential flow cytometric gating strategy used to identify and characterize murine NK cells from single-cell suspensions of spleen tissue. ( a ) Lymphocyte gate: Representative flow cytometry plots illustrating the initial electronic gate set on the small lymphocyte population based on forward scatter (FSC) and side scatter (SSC) characteristics following red blood cell lysis and Fc receptor blocking with 2.4G2 antibody. ( b ) Singlet discrimination: Doublets were excluded by FSC-A versus FSC-H gating to ensure analysis of single cells. ( c ) NK cell identification: Within the lymphocyte gate, NK cells were identified as NK1.1 + cells negative for T cell markers (CD3, TCRβ) and B cell markers (CD19), yielding a purified NK cell population defined as NK1.1 + CD3 − TCRβ − CD19 − cells. CD122 expression was consistently detected within this gate, further confirming NK cell identity. ( d ) Subset characterization: Representative flow cytometric plots showing differential expression of CD127 and additional maturation and receptor markers, such as Mac-1, CD43, Ly49D, NKG2A/C/E, CD27, and CD11c, within the CD127 + and CD127 − NK cell subsets. Numbers in quadrants indicate the percentage of cells within each region.

    Article Snippet: The 2.4G2 hybridoma (anti-FcγRII/III) was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Single Cell, Flow Cytometry, Red Blood Cell Lysis, Blocking Assay, Purification, Expressing, Quantitative Proteomics